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Ensure you meet medical criteria : Make sure that all medical criteria are met, such as BMI thresholds and treatment durations
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(2) DL, Diluted ad libitum diet where animals were provided with ad libitum access to a low-energy diet diluted with indigestible cellulose, which reduced caloric intake by ~30% and (3) CR, animals in which calories were restricted by 30%, and animals were fed once per day during the start of the light period
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